that’s depends for what. If you are asking about a personal trainer certification, then the answer is ‘no’ for most certifying organizations. However, there are some, such as the CSCS certification, that require that you hold a BA.
Again, on the assumption that you are asking about a personal trainer certification, there are some employers that require a college degree in an exercise related field, regardless of which certification you have.
You can get a lot of information on this website when you click on the tabs “Certifications & Trainings” and “Career Guide”.